Premium
ISOLATION AND REGENERATION OF HAPLOID PROTOPLASTS FROM BANGIA ATROPURPUREA (RHODOPHYTA) WITH MARINE BACTERIAL ENZYMES 1
Author(s) -
Araki Toshiyoshi,
Hayakawa Michio,
Tamaru Yutaka,
Yoshimatsu Keiji,
Morishita Tatsuo
Publication year - 1994
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.0022-3646.1994.01040.x
Subject(s) - protoplast , biology , mannitol , botany , frond , isolation (microbiology) , vibrio , xylanase , sporangium , enzyme , bacteria , biochemistry , microbiology and biotechnology , spore , genetics
Three kinds of enzymes, agarase, β‐1,4‐mannanase, and β‐1,3‐xylanase, required for isolation of protoplasts from the red alga Bangia atropurpurea (Roth) C. Ag. were prepared from bacterial culture fluids of Vibrio sp. PO‐303, Vibrio sp. MA‐138, and Alcaligenes sp. XY‐234, respectively, isolated from the sea environment. The optimal pH of all enzymes was around 7.5. Suitable conditions for protoplast isolation from B. atropurpurea were examined. The pretreatment of the fronds with pa‐pain solution (20 mM Mes buffer, pH 7.5, containing 2% papain and 0.5 M mannitol) contributed to successful protoplast isolation. When razor‐cut fragments of the fronds (about 200 mg in fresh weight) immersed in 20 mM Mes buffer, 7.5, containing 0.5 M mannitol and one unit each of agarase, β‐1,4‐mannanase, and β‐1,3‐xylanase were incubated at 22°C for 90 min with gentle agitation, 5.7 × 10 6 protoplasts were released from them. Many protoplasts regenerated into fronds of regular or irregular shape.