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IMMUNOLOCALIZATION OF CENTRIN IN OXYRRHIS MARINA (DINOPHYCEAE) 1
Author(s) -
Höhfeld Ingo,
Beech Peter L.,
Melkonian Michael
Publication year - 1994
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.0022-3646.1994.00474.x
Subject(s) - biology , basal body , flagellum , dinophyceae , pyrenoid , chlorophyta , immunoelectron microscopy , botany , immunogold labelling , flagellate , microbiology and biotechnology , ultrastructure , algae , biochemistry , antibody , ecology , phytoplankton , chloroplast , nutrient , gene , immunology
A new polyclonal antibody was raised against centrin isolated from the flagellate green alga Spermatozopsis similis (Chlorophyta; anti‐SSC). It stains by immunofluorescence and immunoelectron microscopy well‐known reference systems for centrin like the nucleus–basal body connectors in Chlamydomonas reinhardtii (Chlorophyta) and the system II fibers (rhizoplasts) of Scherffelia dubia (Chlorophyta). In addition, it recognizes in immunoblots a single 20‐kDa protein in isolated cytoskeletons of Spermatozopsis similis and Tetraselmis striata (Chlorophyta) as well as purified centrin isolated from Tetraselmis striata. Using this antibody, centrin was localized in whole cells and isolated cytoskeletons of Oxyrrhis marina Dujardin (Dinophyceae) by immunofluorescence and immunogold electron microscopy. In the flagellar apparatus of O. marina , five different structures were antigenic. Four short fibers (connectives 1–4) link the basal bodies to the four major fibrous flagellar roots, which do not cross‐react with anti‐centrin. The most prominent of the labeled structures (connective 5), a crescent‐shaped fiber, extends from the flagellar canal of the transverse flagellum along the base of the tentacle to the flagellar canal of the longitudinal flagellum, interconnecting the distal parts of the microtubular roots/bands in the basal apparatus. For most of its length, it underlies and is connected to a transversely oriented subamphiesmal microtubular band. In immunoblot analyses, anti‐SSC recognizes only a single 20‐kDa protein in cytoskeletons of O. marina. Functional and phylogenetic aspects of centrin‐containing structures in dinoflagellates are discussed.