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APPLICATION OF RAPD MARKERS IN AN EXAMINATION OF HETEROSIS IN GELIDIUM VAGUM (RHODOPHYTA) 1
Author(s) -
Patwary Mohsin U.,
Meer John P.
Publication year - 1994
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.0022-3646.1994.00091.x
Subject(s) - heterosis , biology , hybrid , outcrossing , rapd , gametophyte , thallus , plant reproductive morphology , population , botany , inbred strain , genetics , gene , genetic diversity , pollen , demography , sociology
Four morphologically indistinguishable isolates of monoecious Gelidium vagum Okamura were crossed reciprocally to obtain hybrids for a study on heterosis in this alga. Approximately 50% outcrossing was achieved by adding a small fragment of the designated female parent to a much larger quantity of designated male thallus in the crossing dish. Hybrids in the mixed population of isomorphic hybrid and inbred sporelings were identified by the presence of male‐specific random amplified polymorphic DNA markers. Growth performance of hybrid tetrasporophytes was compared to that of their gametophytic parents and with inbred tetrasporophytes at near‐optimum and sub‐optimum temperature and density. In general, the hybrids showed growth superiority over inbred lines, particularly under sub‐optimum conditions. In these experiments, the hybrid plants from cross 129 × 130 exhibited a 9.5–130% higher growth rate as compared to the mid‐value of the related inbred tetrasporophytes, strongly suggesting the presence of heterosis.