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GENOTYPIC RELATIONSHIPS IN TRICHODESMIUM (CYANOPHYCEAE) BASED ON nifH SEQUENCE COMPARISONS 1
Author(s) -
BenPorath Judith,
Carpenter Edward J.,
Zehr Jonathan P.
Publication year - 1993
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.0022-3646.1993.00806.x
Subject(s) - trichodesmium , biology , cyanobacteria , botany , sequence (biology) , nucleic acid sequence , diazotroph , dna , genetics , nitrogen fixation , bacteria
The DNA sequence of a fragment of nifH was compared to natural populations of the marine cyanobacteria Trichodesmium thiebautii and T. erythraeum from the Caribbean Sea and the unialgal culture Trichodesmium sp. NIBB 1067, which was isolated from the Kuroshio waters (Japan). Through replication Of amplification, cloning, and sequencing, four nucleotides in a 359‐bp fragment were identified that were identical in sequence to Trichodesmium sp. NIBB 1067 and natural populations of T. erythraeum but were distinctly different in sequence from T. thiebautii . The data indicate that Trichodesmium sp. NIBB 1067 is more closely related to T. erythraeum than to T. thiebautii .