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LITHIUM CHLORIDE EXTRACTION OF DNA FROM THE SEAWEED PORPHYRA PERFORATA (RHODOPHYTA) 1
Author(s) -
Hong YongKi,
Coury Daniel A.,
PolneFuller Miriam,
Gibor Aharon
Publication year - 1992
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.0022-3646.1992.00717.x
Subject(s) - porphyra , biology , dna , extraction (chemistry) , centrifugation , biochemistry , algae , ammonium chloride , bromide , dna extraction , chloride , lithium chloride , polysaccharide , chromatography , differential centrifugation , ammonium bromide , phenol , cytoplasm , polymerase chain reaction , botany , gene , chemistry , organic chemistry , pulmonary surfactant
Lithium chloride facilitates the softening of cell walls resulting in a simple, quick (2 h) method for DNA extraction from the red seaweed Porphyra perforata J. Agardh. A 5‐min treatment of tissues in Lid at 55°C extracts DNA that is relatively free of the viscous polysaccharides and proteins that are usually coextracted in large amounts from cell walls and cytoplasm. This protocol does not require grinding of tissues, hydroxyapatite binding, cetyl trimethyl ammonium bromide treatments, enzymatic treatments, phenol extraction, or CsCl‐gradient centrifugation. The resulting DNA is of sufficient quality to be used as a template for polymerase chain reaction amplification.