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COMPARISON OF CHLOROPLAST DNA AND ALLOZYME VARIATION IN WINTER STRAINS OF THE MARINE DIATOM SKELETONEMA COSTATUM (BACILLARIOPHYTA) 1
Author(s) -
Stabile Joseph E.,
Wurtzel Eleanore T.,
Gallagher Jane C.
Publication year - 1992
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.0022-3646.1992.00090.x
Subject(s) - biology , chloroplast dna , restriction fragment length polymorphism , bay , diatom , chloroplast , genetic diversity , restriction fragment , strain (injury) , botany , restriction enzyme , genotype , dna , genetics , gene , population , oceanography , demography , anatomy , geology , sociology
Restriction fragment length polymorphisms (RFLPs) were examined in 12 winter strains of the marine diatom Skeletonema costatum (Grev.) Cleve using homologous chloroplast gene probes. The winter strains included eight different allozyme genotypes exhibiting physiological differences. These 12 winter strains were representative of the least diverse genetic group present in Narragansett Bay populations. Five chloroplast DNA probes and four different restriction enzymes were used to analyze the 12 Narragansett Bay strains and a reference strain “Skel.” A total of 46 restriction fragments were identified. All 12 of the winter strains had identical patterns. Strain Skel exhibited two RFLPs in comparison to the Narragansett Bay strains. Calculated diversity within the winter strain group was 0.0 and 0.85 for the chloroplast DNA and allozyme data, respectively. The chloroplast DNA polymorphisms revealed by this study are expected to represent a minimum level of the chloroplast DNA diversity present in Narragansett Bay seasonal populations.