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A NOVEL TECHNIQUE FOR PREPARATION OF AXENIC CULTURES OF SYMBIODINIUM (PYRROPHYTA) THROUGH SELECTIVE DIGESTION BY AMOEBAE 1
Author(s) -
PolneFuller Miriam
Publication year - 1991
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.0022-3646.1991.00552.x
Subject(s) - biology , axenic , algae , amoeba (genus) , vacuole , digestion (alchemy) , ciliate , microbiology and biotechnology , botany , chlorophyta , symbiodinium , bacteria , biochemistry , ecology , symbiosis , chromatography , chemistry , genetics , cytoplasm
The marine amoeba Trichosphaerium Am‐I‐7 was used as a tool for preparing unialgal axenic cultures of nondigestible Symbiodinium and Porphyridium species. The resistance of these unicellular algae to the amoebal digestive enzymes, and the differential digestion of bacteria, protozoans, and other algae, resulted in cleansed cells of Symbiodinium and Porphyridium that remained in the amoebal food vacuoles. During multiple fission, the amoeba evacuated its food vacuoles and released the trapped and intact algae, which were then successfully cultured. This method of cleaning was especially useful with algal species that were sensitive to antibiotics or other germicidal agents.