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CHANGE OF UBIQUITINATED PROTEINS DURING THE CELL CYCLE IN CHLAMYDOMONAS (CHLOROPHYTA) 1
Author(s) -
Shimogawara Kousuke,
Muto Shoshi
Publication year - 1991
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.0022-3646.1991.00385.x
Subject(s) - biology , chlamydomonas , chlamydomonas reinhardtii , cell cycle , ubiquitin , gamete , aphidicolin , microbiology and biotechnology , dna replication , nuclear protein , hsp70 , heat shock protein , biochemistry , dna , cell , botany , gene , sperm , transcription factor , mutant
Changes in the amount of heat shock‐related ubiquitinated proteins in Chlamydomonas were investigated during the cell cycle and gamete induction. In a division‐synchronized culture induced by periodic illumination, the amount of the 28‐kDa ubiquitinated protein increased during the dark phase. This increase correlated with the increase of total DNA. Such an increase was repressed when nuclear DNA replication was inhibited with aphidicolin. These results suggest that ubiquitination to form the 28‐kDa protein is involved in nuclear DNA replication or during the cell cycle. The amount of 31‐kDa ubiquitinated protein gradually increased throughout the light phase and decreased in the dark phase. The amount of 28‐kDa ubiquitinated protein also increased during gamete induction caused by nitrogen starvation, while that of the 31‐kDa did not. These results suggest that the change of ubiquitination of 28‐kDa protein mat play a fundamental role in the cell cycle and gamete induction in Chlamydomonas .