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COPPER TOLERANCE OF CHLAMYDOMONAS ACIDOPHILA (CHLOROPHYCEAE) ISOLATED FROM ACIDIC, COPPER‐CONTAMINATED SOILS 1
Author(s) -
Twiss Michael R.
Publication year - 1990
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.0022-3646.1990.00655.x
Subject(s) - copper , chlamydomonas reinhardtii , biology , chlorophyceae , strain (injury) , chlamydomonas , botany , microbiology and biotechnology , environmental chemistry , algae , biochemistry , chlorophyta , chemistry , metallurgy , gene , materials science , anatomy , mutant
Cells of Chlamydomonas acidophila Negoro, isolated from three soils with different available copper contents (74, 80, and 87 μg·g −1 ), were assayed for their responses to copper. Soil pH ranged from 3.3–3.9. Responses were evaluated using algistatic assays involving five day exposure to copper concentrations from 0.1–100 mg·L −1 at pH 3.8 and 6.6 in defined liquid media. Interspecies and intraspecies comparisons were made between the soil isolates and laboratory strains of C. reinhardtii and C. acidophila , respectively. Algistatic copper concentrations of soil isolates were 20–125 times greater than those of the laboratory strain of C. reinhardtii. Concentrations of 0.1 mg Cu·L −1 , or greater, killed the laboratory strain of C. acidophila. Soil isolates of C. acidophila appeared to be copper tolerant; however, there was no conclusive evidence to indicate that the level of copper tolerance in the soil isolates was positively correlated with the level of available copper in the soil.