Premium
NUCLEOTIDE SEQUENCE OF THE GENE FOR THE LARGE SUBUNIT OF RIBULOSE‐1.5‐DISPHOSPHATE CARBOXYLASE/OXYGENASE FROM CRYPTOMNAS Φ EVIDENCE SUPPORTING THE POLYPHYLETIC ORGIN OF PLASTIDS 1
Author(s) -
Douglas Susan E.,
Durnford Dion G.,
Modern Clifford W.
Publication year - 1990
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.0022-3646.1990.00500.x
Subject(s) - biology , rubisco , chloroplast dna , nucleic acid sequence , plastid , protein subunit , chloroplast , specificity factor , biochemistry , phycobiliprotein , gene , genetics , microbiology and biotechnology , promoter , cyanobacteria , bacteria , gene expression
The Chloroplast‐encoded gene for the large subunit of ribulose‐1, 5‐bisphosphate carboxylase/oxygenase (Rubisco) from the marine chlorophyll c 2 ‐, phycobiliprotein‐containing alga, Cryptomonas φ was cloned, sequenced and compared to representatives from other groups of photosynthetic organisms. The coding sequence was 1464 base pairs(bp) and encoded a polypeptide of 488 amino acids. The small subunit of Rubisco was located 83 bp 3′ to the large subunit and was co‐transcribed. A 5 bp sequence, 5′ of the initiator methionine codons of both the large and small subunits, may represent ribosomal binging sites. The start of transcription was identified by primer extension studies and putative promotor sequences were found. This study is the first report of the sequence for the large subunit of Rubisco from a chromophyte alga, and its potential as phylogenetic indicator for algal plastids sis discussed.