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KINETICS OF PHOSPHATE TRANSPORT BY SYNECHOCOCCUS LEOPOLIENSIS (CYANOPHYTA): EVIDENCE FOR DIFFUSION LIMITATION OF PHOSPHATE UPTAKE 1
Author(s) -
Mierle Greg
Publication year - 1985
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.0022-3646.1985.00177.x
Subject(s) - kinetics , phosphate , activation energy , synechococcus , diffusion , saturation (graph theory) , enzyme kinetics , biology , diffusion process , biochemistry , thermodynamics , biophysics , enzyme , chemistry , cyanobacteria , physics , active site , genetics , mathematics , quantum mechanics , combinatorics , bacteria , innovation diffusion , knowledge management , computer science
The kinetics of phosphate transport by Synechococcus leopoliensis (Racih.) Komarek was investigated. Deviations from Michaelis‐Menten kinetics were observed at law concentrations of phosphate and the deviations were consistent with diffusion limitation of transport. Activation energy analysis of the transport process at two concentrations, one at carrier saturation and the other at zero added phosphate yielded activation energies of 11.9 and 5.6 kcal/mole respectively at 25° C. The first is consistent with an enzyme limited process and the second with diffusion limitation through the unstirred layer.