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NUCLEAR SYNCHRONY IN VALONIA MACROPHYSA (CHLOROPHYTA): LIGHT MICROSCOPY AND FLOW CYTOMETRY 1
Author(s) -
Staves Mark P.,
Claire John W.
Publication year - 1985
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.0022-3646.1985.00068.x
Subject(s) - flow cytometry , biology , nuclear dna , cell cycle , chlorophyta , algae , biophysics , cell nucleus , microbiology and biotechnology , botany , cell , nucleus , biochemistry , mitochondrial dna , gene
The coenocytic alga Valonia macrophysa Kützing was selected for an investigation of nuclear synchrony in the order Siphonocladales. Light microscopy reveals that nuclear synchrony is evident as patches of nuclei dividing simultaneously. Flow cytometry was utilized for the first time with a macroalga for cell‐cycle analysis. Results indicate that nuclei in the entire cell exhibit a high degree of synchrony throughout the cell cycle. Also it appears that cells within a clonal culture are synchronous with each other, in their progression through the cell cycle. The advantages of using flow cytometry for cell‐cycle analysis of coenocytic algae include the rapid collection of quantitative data on relative DNA content for a large number of nuclei.