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DEMONSTRATION OF NET INFLUX OF FREE AMINO ACIDS IN PHAEODACTYLUM TRICORNUTUM USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY 1
Author(s) -
Lu Ming,
Stephens Grover C.
Publication year - 1984
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.0022-3646.1984.00584.x
Subject(s) - phaeodactylum tricornutum , glycine , high performance liquid chromatography , biology , amino acid , chromatography , algae , biochemistry , botany , chemistry
Influx of glycine from dilute solution in the medium into Phaeodactylum tricornutum (Bohlin) was studied using radiochemical techniques. Comparison of these data with simultaneous measurements of the disappearance of primary amines from the medium by fluorometry indicates that influx of 14 C‐labeled glycine accurately reflects net entry of substrate. High performance liquid chromatography (HPLC) was employed to demonstrate net removal of fourteen different amino acids from dilute solution by P. tricornutum. HPLC was also used to demonstrate net removal of free amino acids naturally present in sea water.