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CHARACTERISTICS OF PHOSPHORUS LIMITATION IN CHLAMYDOMONAS REINHARDTH (CHLOROPHYCEAE) AND ITS PALMELLOIDS 1
Author(s) -
Olsen Ynguar,
Knutsen Gjert,
Lien Torleiv
Publication year - 1983
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.0022-3646.1983.00313.x
Subject(s) - biology , phosphorus , chlamydomonas , dilution , chlorophyceae , chemostat , growth rate , chlamydomonas reinhardtii , phosphate , botany , chlorophyll , polyphosphate , zoology , horticulture , algae , chlorophyta , biochemistry , chemistry , physics , genetics , geometry , organic chemistry , mathematics , mutant , gene , thermodynamics , bacteria
Chlamydomonas reinhardtii Dang, was grown in a chemostat culture under phosphate limitation. The steady state concentration of phosphate was below the detection limit (< 1 μg P/L) in all runs. The cellular content of phosphorus (Q p ), polyphosphate (Q pp ) and chlorophyll a increased with increasing dilution rate, and the growth rate of the alga was described by Q p as well as Q pp in the Droop model. The ratio Q pp /Q p and the activity of alkaline phosphatase were maximal at high and low growth rates, respectively. Palmelloids of Chlamydomonas were found at high dilution rates (D > 0.12 h −1 ) and became attached to the wall of the culture vessel. They differed from the vegetative stage in both chemical composition and growth rate. Their contents of phosphorus and chlorophyll a were low, as in the vegetative cells, which grew at a low growth rate, whereas the ration Q pp /Q p and the activity of alkaline phosphatase were comparable with those of fast growing vegetative cells. The growth rate of the palmelloids was 0.03 h −1 whereas maximum growth rate (μ m ) for the vegetative cells was 0.21 h −1 .