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PHOSPHOENOLPYRUVATE CARBOXYKINASE ACTIVITY IN ASCOPHYLLUM NODOSUM (PHAEOPHYCEAE) 1
Author(s) -
Kerby Nigel W.,
Evans Leonard V.
Publication year - 1983
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.0022-3646.1983.00001.x
Subject(s) - ascophyllum , carboxylation , phosphoenolpyruvate carboxykinase , biology , phosphoenolpyruvate carboxylase , carbon fixation , biochemistry , botany , algae , enzyme , photosynthesis , catalysis
PEP‐dependent 4 CO 2 ‐fixation by extracts of Ascophyllum nodosum (L.) Le Jol. is reported. The carboxylation of PEP is Mn 2+ dependent and ATP is shown to be a product. IDP was found to be less efficient as a phosphate acceptor than ADP and 3‐mercaptopicolinic acid inhibited the carboxylation reaction. Extracts decarboxylated OAA only in the presence of ATP and had high activities of MDH and GOT. This evidence, together with the probable absence of PEPC, PEPCTrP, and PC in A. nodosum extracts, favors the view that PEPCK is responsible for the light‐independent CO 2 ‐fixation observed in this alga.