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CHLORIDE UPTAKE BY ANACYSTIS NIDULANS (CYANOPHYCEAE) 1
Author(s) -
Craig Susan R.,
Budd Kenneth
Publication year - 1979
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.0022-3646.1979.00300.x
Subject(s) - dcmu , darkness , photosynthesis , antimycin a , cyanide , biology , respiration , biochemistry , chloride , biophysics , botany , nuclear chemistry , electron transport chain , chemistry , photosystem ii , inorganic chemistry , organic chemistry
Anacystis nidulans (Richt.) Drouet & Daily (UTEX 625), grown in batch culture with 0.5% CO 2 in air, was supplied with chloride labelled with 36 Cl in light and dark. Uptake in light was stimulated relative to uptake in darkness. A single transport system for Cl − with an apparent K m for Cl − of 0.14 mM was identified. Chloride in the cells reached a maximum value after 30–50 min at 25 C. At this point the internal Cl − concentration was calculated to be 60‐fold the external (0.1 mM) in light and 37‐fold in darkness. DCMU (3‐[3,4‐dichlorophenyl]–1, 1‐dime‐thylurea), at concentrations which abolished photosynthetic O 2 evolution did not inhibit Cl − uptake in light. Carbonyl cyanide m‐chlorophenyl hydrazone (CCCP), at uncoupling concentrations for photosynthesis and dark respiration, strongly inhibited Cl − uptake in light and darkness. N,N'‐dicyclohexyl carbodiimide (DCCD), an energy transfer inhibitor, inhibited light Cl − uptake more slowly than photosynthesis but had no effect on dark Cl − uptake. It is concluded that Cl − uptake in A. nidulans was active in light and darkness, and that ATP was the probable energy source for transport.