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Shear force near‐field optical microscope based on Q ‐controlled bimorph sensor for biological imaging in liquid
Author(s) -
LEI F. H.,
ANGIBOUST J.F.,
QIAO W.,
SOCKALINGUM G. D.,
DUKIC S.,
CHRIT L.,
TROYON M.,
MANFAIT M.
Publication year - 2004
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.0022-2720.2004.01424.x
Subject(s) - bimorph , cantilever , sensitivity (control systems) , non contact atomic force microscopy , shear force , microscope , resolution (logic) , force dynamics , optics , materials science , microscopy , chemistry , acoustics , physics , kelvin probe force microscope , piezoelectricity , computer science , mechanical engineering , electronic engineering , engineering , composite material , artificial intelligence
Summary Shear force near‐field microscopy on biological samples in their physiological environment loses considerable sensitivity and resolution as a result of liquid viscous damping. Using a bimorph‐based cantilever sensor incorporating force feedback, as recently developed by us, gives an alternative force detection scheme for biological imaging in liquid. The dynamics and sensitivity of this sensor were theoretically and experimentally discussed. Driving the bimorph cantilever close to its resonance frequency with appropriate force feedback allows us to obtain a quality factor ( Q ‐factor) of up to 10 3 in water, without changing its intrinsic resonance frequency and spring constant. Thus, the force detection sensitivity is improved. Shear force imaging on mouse brain sections and human skin tissues in liquid with an enhanced Q ‐factor of 410 have shown a high sensitivity and stability. A resolution of about 50 nm has been obtained. The experimental results suggest that the system is reliable and particularly suitable for biological cell imaging in a liquid environment.