Premium
Automatic real‐time three‐dimensional cell tracking by fluorescence microscopy
Author(s) -
RABUT G.,
ELLENBERG J.
Publication year - 2004
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.0022-2720.2004.01404.x
Subject(s) - tracking (education) , live cell imaging , focus (optics) , microscopy , fluorescence microscope , fluorescence lifetime imaging microscopy , throughput , temporal resolution , fluorescence , microscope , materials science , optics , cell , computer science , computer vision , chemistry , physics , psychology , telecommunications , pedagogy , biochemistry , wireless
Summary Live cell imaging has become an indispensable technique for cell biologists. However, when grown on coverslip glass used for live cell imaging many cultured cells move even during relatively short observation times and focus can drift as a result of mechanical instabilities and/or temperature fluctuations. Time‐lapse imaging therefore requires constant adjustment of the imaging field and focus position to keep the cell of interest centred in the imaged volume. We show here that this limitation can be overcome by tracking cells in a fully automated way using the mass centre of cellular fluorescence. Combined with automated multiple location revisiting, this method dramatically increases the throughput of high‐resolution live cell imaging experiments.