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GFP is the way to glow: bioimaging of the plant endomembrane system
Author(s) -
Brandizzi F.,
Irons S. L.,
Johansen J.,
Kotzer A.,
Neumann U.
Publication year - 2004
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.0022-2720.2004.01334.x
Subject(s) - endomembrane system , green fluorescent protein , bimolecular fluorescence complementation , microbiology and biotechnology , secretory pathway , plant cell , secretory protein , fluorescence , biology , nanotechnology , biophysics , chemistry , secretion , biochemistry , physics , materials science , endoplasmic reticulum , gene , quantum mechanics , golgi apparatus
Summary It is less than a decade that the green fluorescent protein (GFP) and its spectral variants have changed the approach to studying the dynamics of the plant secretory pathway. GFP technology has in fact shed new light on secretory events by allowing bioimaging in vivo right to the heart of a plant cell. This review highlights exciting discoveries and the most recent developments in the understanding of morphology and dynamics of the plant secretory pathway achieved with the application of fluorescent proteins.