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Linear phase imaging using differential interference contrast microscopy
Author(s) -
Arnison M. R.,
Larkin K. G.,
Sheppard C. J. R.,
Smith N. I.,
Cogswell C. J.
Publication year - 2004
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.0022-2720.2004.01293.x
Subject(s) - differential interference contrast microscopy , phase (matter) , optics , interference (communication) , isotropy , fourier transform , microscopy , imaging phantom , contrast (vision) , k space , materials science , phase contrast imaging , amplitude , physics , phase contrast microscopy , computer science , channel (broadcasting) , computer network , quantum mechanics
Summary We propose an extension to Nomarski differential interference contrast microscopy that enables isotropic linear phase imaging. The method combines phase shifting, two directions of shear and Fourier‐space integration using a modified spiral phase transform. We simulated the method using a phantom object with spatially varying amplitude and phase. Simulated results show good agreement between the final phase image and the object phase, and demonstrate resistance to imaging noise.