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Expression of amyloid precursor protein‐like molecule in astroglial cells of the subventricular zone and rostral migratory stream of the adult rat forebrain
Author(s) -
Yasuoka Katsunori,
Hirata Kazuho,
Kuraoka Akio,
He Jianwen,
Kawabuchi Masaru
Publication year - 2004
Publication title -
journal of anatomy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 118
eISSN - 1469-7580
pISSN - 0021-8782
DOI - 10.1111/j.0021-8782.2004.00320.x
Subject(s) - rostral migratory stream , subventricular zone , neuroblast , glial fibrillary acidic protein , neural cell adhesion molecule , biology , olfactory bulb , microbiology and biotechnology , ependymal cell , amyloid precursor protein , neurogenesis , subependymal zone , forebrain , astrocyte , neural stem cell , neuroscience , pathology , immunohistochemistry , cell , stem cell , cell adhesion , central nervous system , immunology , biochemistry , alzheimer's disease , medicine , disease
In adult mammals, new neurons in the subventricular zone (SVZ) of the lateral ventricle (LV) migrate tangentially through the rostral migratory stream (RMS) to the olfactory bulb (OB), where they mature into local interneurons. Using a monoclonal antibody for the β‐amyloid precursor protein (APP) (mAb 22C11), which is specific for the amino‐terminal region of the secreted form of APP and recognizes all APP isoforms and APP‐related proteins, immunoreactivity was detected in specific subpopulations of cells in the SVZ and RMS of the adult rat forebrain. In the SVZ, APP‐like immunoreactivity was detected in the ependymal cells lining the LV and some of the subependymal cells. The latter were regarded as astrocytes, because they were positive for the glial markers, S‐100 protein (S‐100) and glial fibrillary acidic protein (GFAP). APP‐like immunoreactive astrocytes exhibited strong labelling of the perinuclear cytoplasm and often possessed a long, fine process similar to that found with radial glia. The process extended to an APP‐like immunoreactive meshwork in the RMS that consisted of cytoplasmic processes of astrocytes forming ‘glial tubes’. Double‐immunofluorescent labelling with a highly polysialylated neural cell adhesion molecule (PSA‐NCAM) confirmed that the APP‐like immunoreactive astrocytes in the SVZ and meshwork in the RMS made close contact with PSA‐NCAM‐immunopositive neuroblasts, suggesting an interaction between APP‐containing cells and neuroblasts. This region of the adult brain is a useful in vivo model to investigate the role of APP in neurogenesis.