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CD28 is not directly involved in the response of human CD3 − CD56 + natural killer cells to lipopolysaccharide: a role for T cells
Author(s) -
Goodier M. R.,
Londei M.
Publication year - 2004
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/j.0019-2805.2004.01834.x
Subject(s) - cd28 , cytotoxic t cell , interleukin 21 , biology , cd3 , peripheral blood mononuclear cell , natural killer t cell , monoclonal antibody , il 2 receptor , microbiology and biotechnology , lymphokine activated killer cell , antibody , natural killer cell , antigen , lipopolysaccharide , immunology , t cell , immune system , cd8 , in vitro , biochemistry
Summary We have previously shown that human CD3 − CD56 + and CD3 + CD56 + cells from some individuals mount vigorous proliferative responses to lipopolysaccharide. Such responses have been blocked by the presence of cytotoxic T‐lymphocyte antigen‐4 immunoglobulin fusion protein in the cultures, implicating a role for B7‐mediated costimulation. Here we confirm this inhibition of natural killer (NK) expansion using antibodies against B7‐1 and B7‐2. We were unable to specifically detect CD28 on the surface of resting or stimulated human peripheral blood NK cells, however, in either lipopolysaccharide‐responsive or non‐responsive individuals, using a panel of four different anti‐CD28 monoclonal antibodies. T‐cell depletion from peripheral blood mononuclear cell cultures resulted in a reduction in the induction of CD25 on activated CD3 − CD56 hi cells and in the expansion and proliferation of CD3 − CD56 + NK cells. Furthermore, reconstitution experiments using peripheral blood dendritic cells and purified NK cells demonstrated that NK expansion could only be achieved in the presence of purified T cells.