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Sub‐cellular localization of Ni in the hyperaccumulator, Hybanthus floribundus (Lindley) F. Muell
Author(s) -
BIDWELL S. D.,
CRAWFORD S. A.,
WOODROW I. E.,
SOMMERKNUDSEN J.,
MARSHALL A. T.
Publication year - 2004
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.0016-8025.2003.01170.x
Subject(s) - hyperaccumulator , vacuole , apoplast , microanalysis , chemistry , botany , biophysics , biology , cell wall , biochemistry , environmental chemistry , cytoplasm , heavy metals , organic chemistry , phytoremediation
The cellular and subcellular distribution of Ni within leaves of Hybanthus floribundus (Lindley) F. Muell, a hyperaccumulator of Ni, was investigated at relatively high spatial resolution using energy‐dispersive X‐ray microanalysis (EDAX). Elemental distribution maps showed that Ni was predominantly localized in the vacuoles of epidermal cells in the leaves. Quantification of Ni revealed concentrations up to 275 mmol kg −1 (embedded tissue) in some epidermal vacuoles. The accumulation of Ni in these cells was associated with a decrease in the concentration of Na and K. There was no indication that Ni was associated with P, S or Cl in the vacuoles. Ni was also concentrated on the outside of cell walls throughout the leaves, indicating that apoplastic compartmentation is also involved in Ni tolerance and accumulation in this plant.