Open AccessStructure of the fructose‐containing K52 capsular polysaccharide of uropathogenic Escherichia coli O4:K52:H −
Author(s) -
HOFMANN Peter,
JANN Barbara,
JANN Klaus
Publication year - 1985
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.0014-2956.1985.00601.x
Subject(s) - polysaccharide , phosphodiester bond , galactose , chemistry , periodate , escherichia coli , hydrolysis , biochemistry , acetylation , fructose , oligosaccharide , acid hydrolysis , nuclear magnetic resonance spectroscopy , anomer , stereochemistry , rna , gene
The chemical structure of the K52 antigenic capsular polysaccharide (K52 antigen) of Escherichia coli O4:K52:H − was elucidated by composition, nuclear magnetic resonance spectroscopy, methylation, periodate oxidation before and after graded acid hydrolysis and by oligosaccharide analysis. The polysaccharide consists of a backbone of α‐galactose units interlinked between C1 and C3 by phosphodiester bridges. This poly(α‐galactosyl‐phosphate) is substituted at C2 of each galactose unit by β‐fructofuranose residues. About 80% of the galactose units are O ‐acetylated at C4 and about 10% of the fructose units are both O ‐acetylated and O ‐propionylated at C1. The K52 polysaccharide has an average molecular mass of 34 kDa, thus consisting of approximately 65 fructosyl‐galactosyl‐phosphate repeating units.