Open AccessIdentification of 2‐guanidinoethanol in human urine
Author(s) -
WATANABE Yoko,
SHINDO Shoichiro,
MORI Akitane
Publication year - 1985
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.0014-2956.1985.00465.x
Subject(s) - urine , chemistry , chromatography , derivative (finance) , mass spectrometry , trifluoroacetic acid , creatinine , biochemistry , financial economics , economics
Guanidino compounds in normal human urine were analyzed by high‐performance liquid chromatography; an unknow peak was observed in the chromatogram that was identical to the peak of synthetic 2‐guanidinoethanol. In another experiment, the substance was purified from human urine by successive use of strongly acidic ion‐exchanger, thin‐layer chromatography and then weakly acidic ion‐exchanger. After this it was reacted with acetylacetone to form dimethylpyrimidyl derivative. After further reaction of this derivative with trifluoroacetic anhydrate, it was analyzed by gas chromatography/mass spectrometry. The mass chromatogram and mass spectrum were identical to those of the trifluoroacetylated dimethylpyrimidyl derivative of synthetic 2‐guanidinoethanol. This is the first report on the identification of 2‐guanidinoethanol in human urine. The concentration of 2‐guanidinoethanol in the urine of healthy humans was 5.7 ± 1.8 (mean ± SD) μmol1/g creatinine.