Open AccessCombination of resveratrol‐containing collagen with adipose stem cells for craniofacial tissue‐engineering applications
Author(s) -
Wang ChihChien,
Wang ChihHsin,
Chen HsiangCheng,
Cherng JuinHong,
Chang ShuJen,
Wang YiWen,
Chang Adrienne,
Yeh JueZong,
Huang YiHuei,
Liu ChengChe
Publication year - 2018
Publication title -
international wound journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.867
H-Index - 63
eISSN - 1742-481X
pISSN - 1742-4801
DOI - 10.1111/iwj.12910
Subject(s) - regeneration (biology) , mesenchymal stem cell , scaffold , craniofacial , stem cell , adipose tissue , wound healing , microbiology and biotechnology , tissue engineering , biomedical engineering , medicine , pathology , anatomy , immunology , biology , psychiatry
Repair and regeneration of craniofacial tissues is particularly challenging because they comprise a complex structure of hard and soft tissues involved in intricate functions. This study combined collagen scaffolds and human adipose stem cells (hASCs) for oral mucosal and calvarial bone regeneration by using resveratrol (RSV), which affects the differentiation of mesenchymal stem cells. We have evaluated the effect of collagen scaffold‐containing RSV (collagen/RSV) scaffolds both in vitro and in vivo for their wound healing and bone regeneration potential. Scanning electron microscopy and immunostaining results reveal that hASCs adhere well to and proliferate on both collagen scaffolds and collagen/RSV scaffolds. Oral mucosal lesion experiments demonstrated that the collagen/RSV scaffold is more effective in wound closure and contraction than the collagen scaffold. The micro‐computed tomography (μCT) images of calvarial bone display regenerating bone in defects covered with hASCs on collagen/RSV scaffolds that are more visible than that in defects covered with hASCs on a collagen scaffolds. RSV was more effective at inducing hASC differentiation on the collagen scaffold, suggesting that collagen/RSV scaffolds can provide useful biological cues that stimulate craniofacial tissue formation.