Open AccessMesenchymal stem cell‐conditioned medium accelerates wound healing with fewer scars
Author(s) -
Li Meirong,
Luan Fuxin,
Zhao Yali,
Hao Haojie,
Liu Jiejie,
Dong Liang,
Fu Xiaobing,
Han Weidong
Publication year - 2017
Publication title -
international wound journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.867
H-Index - 63
eISSN - 1742-481X
pISSN - 1742-4801
DOI - 10.1111/iwj.12551
Subject(s) - mesenchymal stem cell , scars , paracrine signalling , wound healing , medicine , myofibroblast , matrix metalloproteinase , fibroblast , dermal fibroblast , pathology , cancer research , in vitro , immunology , fibrosis , biology , biochemistry , receptor
Mesenchymal stem cells ( MSCs ) derived from umbilical cord s ( UC‐MSCs ) have been shown to enhance cutaneous wound healing by means of the paracrine activity. Fibroblasts are the primary cells involved in wound repair. The paracrine effects of UC‐MSCs on dermal fibroblasts have not been fully explored in vitro or in vivo. Dermal fibroblasts were treated with conditioned media from UC‐MSCs ( UC‐MSC‐CM ). In this model, UC‐MSC‐CM increased the proliferation and migration of dermal fibroblasts. Moreover, adult dermal fibroblasts transitioned into a phenotype with a low myofibroblast formation capacity, a decreased ratio of transforming growth factor‐β1,3 (TGF‐β1/3) and an increased ratio of matrix metalloproteinase/tissue inhibitor of metalloproteinases (MMP/TIMP). Additionally, UC‐MSC‐CM ‐treated wounds showed accelerated healing with fewer scars compared with control groups. These observations suggest that UC‐MSC‐CM may be a feasible strategy to promote cutaneous repair and a potential means to realise scarless healing.