Open AccessImmunomodulator ‘mushroom beta glucan’ induces Wnt/β catenin signalling and improves wound recovery in tilapia and rat skin: a histopathological study
Author(s) -
Hsiao ChienMei,
Wu YuSheng,
Nan FanHua,
Huang ShihLing,
Chen Lynette,
Chen ShiuNan
Publication year - 2016
Publication title -
international wound journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.867
H-Index - 63
eISSN - 1742-481X
pISSN - 1742-4801
DOI - 10.1111/iwj.12427
Subject(s) - bromodeoxyuridine , wound healing , viability assay , in vivo , medicine , cell growth , in vitro , wnt signaling pathway , oreochromis , andrology , pharmacology , pathology , immunohistochemistry , biology , immunology , microbiology and biotechnology , biochemistry , signal transduction , fishery , fish <actinopterygii>
The present study aims to investigate the effects of mushroom beta glucan (MBG) on wound recovery in partial hepatectomy (PH) in Nile tilapia ( Oreochromis niloticus ) and in rat skin wound healing examination. Following PH, we focussed on the effects on liver repair ability using in vitro and in vivo tests. In vitro, we examined whether the MBG has an impact on liver cell proliferation, mainly through 3‐(4,5‐dimethylthiazolyl‐2)‐2,5‐diphenyltetrazolium bromide ( MTT ) assays and bromodeoxyuridine ( BrdU ) cell proliferation assay detection method. Results showed that MBG treatment was remarkable in enhancing cell proliferation of hepatocytes and in maintaining the cellular viability. Immunohistochemical staining to analyse Wnt/β‐catenin signalling also showed that MBG has the effect of promoting cell proliferation of liver tissues after PH surgery.