Open AccessPreliminary investigation of topical nitroglycerin formulations containing natural wound healing agent in diabetes‐induced foot ulcer
Author(s) -
Hotkar Mukesh S,
Avachat Amelia M,
Bhosale Sagar S,
Oswal Yogesh M
Publication year - 2015
Publication title -
international wound journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.867
H-Index - 63
eISSN - 1742-481X
pISSN - 1742-4801
DOI - 10.1111/iwj.12084
Subject(s) - aloe vera , medicine , wound healing , ex vivo , diabetic foot ulcer , in vivo , diabetic foot , diabetes mellitus , pharmacology , surgery , traditional medicine , microbiology and biotechnology , biology , endocrinology
Nitroglycerin ( NTG ) is an organic nitrate rapidly denitrated by enzymes to release free radical nitric oxide and shows improved wound healing and tissue protection from oxidative damage. The purpose of this study was to evaluate whether topical application of NTG in the form of gel/ointment along with a natural wound healing agent, aloe vera, would bring about wound healing by using diabetes‐induced foot ulcer model and rat excision wound model. All these formulations were evaluated for pH , viscosity, drug content and ex vivo diffusion studies using rat skin. Based on ex vivo permeation studies, the formulation consisting of carbopol 974p as a gelling agent and aloe vera was found to be suitable. The in vivo study used streptozotocin‐induced diabetic foot ulcer and rat excision wound models to analyse wound healing activity. The wound size in animals of all treated groups was significantly reduced compared with that of the diabetic control and marketed treated animals. This study showed that the gel formed with carbopol 974p (1%) and aloe vera promotes significant wound healing and closure in diabetic rats compared with the commercial product and provides a promising product to be used in diabetes‐induced foot ulcer.