Open AccessInhibition of biofilms by glucose oxidase, lactoperoxidase and guaiacol: the active antibacterial component in an enzyme alginogel
Author(s) -
Cooper Rose A
Publication year - 2013
Publication title -
international wound journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.867
H-Index - 63
eISSN - 1742-481X
pISSN - 1742-4801
DOI - 10.1111/iwj.12083
Subject(s) - guaiacol , lactoperoxidase , glucose oxidase , biofilm , enzyme , medicine , antibacterial activity , biochemistry , microbiology and biotechnology , component (thermodynamics) , bacteria , chemistry , peroxidase , biology , genetics , physics , thermodynamics
The association of biofilms with wound chronicity has prompted a search for antimicrobial interventions that are effective against biofilms. A patented preparation of glucose oxidase, lactoperoxidase and guaiacol ( GLG ), which is the antibacterial component of Flaminal®, has been shown to inhibit a wide range of bacteria, but it has not yet been tested on biofilms. This study aims to determine the effect of GLG on biofilms of Staphylococcus aureus , methicillin‐resistant S. aureus and Pseudomonas aeruginosa . Static biofilms were grown in microtitre plates and on coverslips and treated with a range of concentrations of GLG . Effects were monitored by estimating biofilm biomass by staining with crystal violet, biofilm activity by staining with either resazurin or fluorescein diacetate and biofilm viability by staining with LIVE / DEAD ® BacLight ™ Bacterial Viability Kit. GLG was able to prevent the formation of biofilms at concentration ≤0·5% (w/v) and higher concentrations were required to inhibit established biofilms. GLG did not disrupt biofilm biomass. Staphylococci were more susceptible to GLG than P. aeruginosa . These in vitro findings must be verified by in vivo studies.