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Transcriptional levels of the cancer‐related genes p53 , TAp63/73, and ∆Np63/73 in the blue mussel ( Mytilus edulis ) after lead exposure
Author(s) -
Guerreiro Amanda da Silveira,
Rola Regina C.,
Almeida Daniela V.,
Sandrini Juliana Z.
Publication year - 2018
Publication title -
invertebrate biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.486
H-Index - 42
eISSN - 1744-7410
pISSN - 1077-8306
DOI - 10.1111/ivb.12205
Subject(s) - biology , gill , mytilus , gene isoform , mussel , blue mussel , downregulation and upregulation , gene , transcriptome , microbiology and biotechnology , gene expression , genetics , ecology , fishery , fish <actinopterygii>
The major role of the p53 gene family is related to the maintenance of genetic stability, protecting organisms from a wide range of stressors. Considering the importance of p53 in cancer development, this study aimed to analyze the expression of p53 isoforms in the mussel Mytilus edulis after lead exposure. We detected the p53 isoform both in the gills and in the digestive gland of mussels, but detected TAp63/73 and ∆Np63/73 isoforms only in the gills. Lead exposure induced upregulation of p53 mRNA levels in the digestive gland, without any effect in the gills. Conversely, mRNA levels of the TA p63/73 and ∆Np63/73 isoforms were downregulated in the gills after 1 d of exposure to lead. These results suggest that some similarities may exist between vertebrates and invertebrates and, specifically, that the balance between isoforms may represent a crucial step in cellular homeostasis. Moreover, the transcription of these cancer‐related genes seems to be regulated in a tissue‐ and time‐specific manner.