Open AccessEvaluation of bioaerosol samplers for the detection and quantification of influenza virus from artificial aerosols and influenza virus–infected ferrets
Author(s) -
Bekking Christian,
Yip Lily,
Groulx Nicolas,
Doggett Nathan,
Finn Mairead,
Mubareka Samira
Publication year - 2019
Publication title -
influenza and other respiratory viruses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.743
H-Index - 57
eISSN - 1750-2659
pISSN - 1750-2640
DOI - 10.1111/irv.12678
Subject(s) - bioaerosol , indoor bioaerosol , virus , aerosolization , influenza a virus , virology , orthomyxoviridae , environmental science , aerosol , medicine , microbiology and biotechnology , veterinary medicine , environmental chemistry , biology , chemistry , inhalation , organic chemistry , anatomy
Background Bioaerosol sampling devices are necessary for the characterization of infectious bioaerosols emitted by naturally‐infected hosts with acute respiratory virus infections. Assessment of these devices under multiple experimental conditions will provide insight for device use. Objectives The primary objective of this study was to assess and compare bioaerosol sampling devices using a) an in vitro, environmentally‐controlled artificial bioaerosol system at a range of different RH conditions and b) an in vivo bioaerosol system of influenza virus‐infected ferrets under controlled environmental conditions. Secondarily, we also sought to examine the impact of NSAIDs on bioaerosol emission in influenza virus‐infected ferrets to address its potential as a determinant of bioaerosol emission. Methods We examined the performance of low and moderate volume bioaerosol samplers for the collection of viral RNA and infectious influenza virus in vitroand in vivo using artificial bioaerosols and the ferret model of influenza virus infection. The following samplers were tested: the polytetrafluoroethylene filter (PTFE filter), the 2‐stage National Institute of Occupational Safety and Health cyclone sampler (NIOSH cyclone sampler) and the 6‐stage viable Andersen impactor (Andersen impactor). Results The PTFE filter and NIOSH cyclone sampler collected similar amounts of viral RNA and infectious virus from artificially‐generated aerosols under a range of relative humidities (RH). Using the ferret model, the PTFE filter, NIOSH cyclone sampler and the Andersen impactor collected up to 3.66 log 10 copies of RNA/L air, 3.84 log 10 copies of RNA/L air and 6.09 log 10 copies of RNA/L air respectively at peak recovery. Infectious virus was recovered from the PTFE filter and NIOSH cyclone samplers on the peak day of viral RNA recovery. Conclusion The PTFE filter and NIOSH cyclone sampler are useful for influenza virus RNA and infectious virus collection and may be considered for clinical and environmental settings.