Open AccessComparison of hemagglutination inhibition, single radial hemolysis, virus neutralization assays, and ELISA to detect antibody levels against seasonal influenza viruses
Author(s) -
Trombetta Claudia Maria,
Remarque Edmond J.,
Mortier Daniella,
Montomoli Emanuele
Publication year - 2018
Publication title -
influenza and other respiratory viruses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.743
H-Index - 57
eISSN - 1750-2659
pISSN - 1750-2640
DOI - 10.1111/irv.12591
Subject(s) - hemagglutination assay , neutralization , serology , virology , antibody , hemolysis , virus , influenza a virus , biology , hemagglutination , microbiology and biotechnology , immunology , titer
Background The immunological response to influenza vaccine and/or natural infection is evaluated by serological techniques, the most common being hemagglutination inhibition (HI), single radial hemolysis (SRH), and virus neutralization assays, which is commonly used in a micro‐neutralization (MN) format. ELISA is not officially required; however, this assay is able to measure different class‐specific antibodies. The four assays identify different sets or subsets of antibodies. Objectives The aim of this study was to establish the correlation among four serological assays using four seasonal influenza strains. Methods The HI, SRH, MN assays, and ELISA were performed on four seasonal influenza strains. Results A strong positive correlation was found between HI and MN and between SRH and MN assays for influenza A strains. The B strains also showed good correlations among the three assays. A positive correlation was also found between ELISA and the “classical” assays for all strains. Concerning the correlates of protection, as defined by HI ≥ 40 and SRH ≥ 25 mm 2 , good agreement was observed for the influenza A strains. By contrast, the agreement for the B strains was very low. Conclusions There is a positive strong correlation among the four serological assays for both A and B strains, especially for the HI and MN assays. There is good agreement on correlates of protection between HI and SRH assays for the A strains, but very low agreement for the B strains, suggesting higher sensitivity of SRH than HI assay in detecting antibodies against the influenza B viruses.