Impact of a large deletion in the neuraminidase protein identified in a laninamivir‐selected influenza A/Brisbane/10/2007 (H3N2) variant on viral fitness  in vitro  and in ferrets | Zendy

Ann Julie | Zendy; Abed Yacine | Zendy; Beaulieu Edith | Zendy; Bouhy Xavier | Zendy; Joly MarieHélène | Zendy; Dubé Karen | Zendy; Carbonneau Julie | Zendy; Hamelin MarieEve | Zendy; Mallett Corey | Zendy; Boivin Guy | Zendy

Open Access

Impact of a large deletion in the neuraminidase protein identified in a laninamivir‐selected influenza A/Brisbane/10/2007 (H3N2) variant on viral fitness in vitro and in ferrets

Author(s) -

Ann Julie,

Abed Yacine,

Beaulieu Edith,

Bouhy Xavier,

Joly MarieHélène,

Dubé Karen,

Carbonneau Julie,

Hamelin MarieEve,

Mallett Corey,

Boivin Guy

Publication year - 2016

Publication title -

influenza and other respiratory viruses

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.743

H-Index - 57

eISSN - 1750-2659

pISSN - 1750-2640

DOI - 10.1111/irv.12356

Subject(s) - neuraminidase , virology , infectivity , biology , zanamivir , virus , viral replication , hemagglutinin (influenza) , in vitro , influenza a virus , medicine , covid-19 , infectious disease (medical specialty) , disease , pathology , biochemistry

Viral fitness of a laninamivir‐selected influenza A/Brisbane/10/2007‐like (H3N2) isolate (LRVp9) containing a 237‐amino acid neuraminidase deletion and a P194L hemagglutinin mutation was evaluated in vitro and in ferrets. LRVp9 and the wild‐type (WT) virus showed comparable replication kinetics in MDCK‐ST6GalI cells. Cultured virus was recovered between days 2 and 5 post‐infection in nasal washes (NW) from the 4 WT‐infected ferrets whereas no virus was recovered from the LRVp9‐infected animals. There was a ≥1 log reduction in viral RNA copies/μl of NW for LRVp9 compared to WT at most time points. The large neuraminidase deletion compromises viral infectivity in vivo .

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