Open AccessHeat inactivation of the M iddle E ast respiratory syndrome coronavirus
Author(s) -
Leclercq India,
Batéjat Christophe,
Burguière Ana M.,
Manuguerra JeanClaude
Publication year - 2014
Publication title -
influenza and other respiratory viruses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.743
H-Index - 57
eISSN - 1750-2659
pISSN - 1750-2640
DOI - 10.1111/irv.12261
Subject(s) - infectivity , vero cell , coronavirus , virology , middle east respiratory syndrome coronavirus , titer , covid-19 , microbiology and biotechnology , betacoronavirus , respiratory system , biology , chemistry , virus , medicine , infectious disease (medical specialty) , outbreak , disease
The culture supernatants of the emerging Middle East respiratory syndrome coronavirus ( MERS ‐CoV) were submitted to three temperatures over time and tested for infectivity by TCID 50 method on Vero E6 cells. At 56°C, almost 25 minutes were necessary to reduce the initial titre by 4 log 10 . Increasing temperature to 65°C had a strong negative effect on viral infectivity as virucidy dropped significantly to 1 minute. On the contrary, no significant decrease in titre was observed after 2 hours at 25°C. These data might be useful in establishing biosafety measures in laboratories against MERS‐CoV.