Open AccessGenetic characterization of influenza virus circulating in Brazilian pigs during 2009 and 2010 reveals a high prevalence of the pandemic H1N1 subtype
Author(s) -
Rajão Daniela S.,
Costa Adrienny T. R.,
Brasil Bruno S. A. F.,
Del Puerto Helen L.,
Oliveira Fernanda G.,
Alves Fabiana,
Braz Gissandra F.,
Reis Jenner K. P.,
Guedes Roberto M. C.,
Lobato Zélia I. P.,
Leite Rômulo C.
Publication year - 2013
Publication title -
influenza and other respiratory viruses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.743
H-Index - 57
eISSN - 1750-2659
pISSN - 1750-2640
DOI - 10.1111/irv.12072
Subject(s) - virology , neuraminidase , hemagglutinin (influenza) , outbreak , pandemic , biology , phylogenetic tree , virus , h5n1 genetic structure , transmission (telecommunications) , influenza a virus , veterinary medicine , gene , medicine , disease , genetics , covid-19 , infectious disease (medical specialty) , pathology , electrical engineering , engineering
Background Influenza A viruses circulating in pigs in Brazil are still not characterized, and only limited data are available about swine influenza epidemiology in the country. Therefore, we characterized the hemagglutinin ( HA ) and neuraminidase ( NA ) genes of influenza viruses isolated from Brazilian pigs. We also evaluated one case of probable swine‐to‐human transmission. Methods Twenty influenza viruses isolated from pigs during 2009–2010 in five Brazilian states (Minas Gerais, Sao Paulo, Parana, Rio Grande do Sul, and Mato Grosso) were used. One human isolate, from a technician who became ill after visiting a swineherd going through a respiratory disease outbreak, was also used in the study. Phylogenetic analysis for the HA and NA genes and hemagglutinin amino acid sequence alignment were performed. Results All isolates clustered with pandemic H1N1 2009 (pH1N1) viruses and appeared to have a common ancestor. Genetic diversity was higher in the HA than in the NA gene, and the amino acid substitution S203T in one of HA 's antigenic sites was found in most of the samples. The human isolate was more related to swine isolates from the same herd visited by the technician than to other human isolates, suggesting swine‐to‐human transmission. Conclusion Our results show that pH 1N1 was disseminated and the predominant subtype in Brazilian pigs in 2009–2010.