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Odontoblast markers and dentine reactions in carious primary molars with and without hypomineralised enamel defects
Author(s) -
Shah Janita,
Manton David J.,
McCullough Michael J.,
Rajan Sadna
Publication year - 2021
Publication title -
international journal of paediatric dentistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.183
H-Index - 62
eISSN - 1365-263X
pISSN - 0960-7439
DOI - 10.1111/ipd.12750
Subject(s) - odontoblast , dmp1 , molar , pulp (tooth) , medicine , enamel paint , dentistry , dentin , viral matrix protein , virus , virology
Background Wnt/β‐Catenin signalling and DMP1 have key roles in tertiary dentinogenesis. Aim To compare the relationship between remaining dentine thickness (RDT), tertiary dentine thickness (TDT), β‐catenin and dentine matrix protein 1 (DMP1) in carious second primary molar teeth with normal (SPM) and hypomineralised enamel (HSPM). Design Extracted carious SPM and HSPM were fixed, sectioned (5 μm) and stained with haematoxylin and eosin or with indirect immunofluorescence for β‐catenin and DMP1. Image analysis was performed to determine RDT, TDT, β‐catenin and DMP1 intensity in the odontoblast layer and dentine‐pulp complex. Results Carious SPM (n = 11; mean RDT = 1536.1 μm) and HSPM (n = 12; mean RDT = 1179.9 μm) had mean TDT 248.6 μm and 518.1 μm, respectively ( P = .02). There were no significant differences in intensity values in the odontoblast layer and dentine‐pulp complex for β‐catenin and DMP1 for both groups. Conclusion There was no observable variation in Wnt/β‐catenin and DMP1 expression between HSPM and SPM despite a statistically significant twofold increased TDT in HSPM compared with SPM that had similar RDT. Thus, the observed increased TDT in HSPM is more likely due to an earlier onset of repair processes rather than an amplified response to caries.