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Carbonic anhydrase VI activity in saliva and biofilm can predict early childhood caries: A preliminary study
Author(s) -
de Sousa Emerson Tavares,
LimaHolanda Aline Tavares,
NobredosSantos Marinês
Publication year - 2021
Publication title -
international journal of paediatric dentistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.183
H-Index - 62
eISSN - 1365-263X
pISSN - 0960-7439
DOI - 10.1111/ipd.12717
Subject(s) - saliva , biofilm , medicine , logistic regression , early childhood caries , carbonic anhydrase , dentistry , microbiology and biotechnology , oral health , chemistry , biochemistry , biology , bacteria , enzyme , genetics
Abstract Aim This study aimed to investigate whether carbonic anhydrase VI activity (CA VI ACT ), pH, and buffering capacity (BC) in saliva and biofilm could predict the number of lesion occurrence and early childhood caries (ECC). Design A cross‐sectional study was performed in a sample of 44 children aged 4 to 5 years who were examined regarding their caries status (dmfs + active white spot lesions–WSL) and allocated into two groups: ECC and caries‐free (CF). Saliva and biofilm were collected to determine pH, BC, and CA VI ACT . Data were analyzed using the Student t test, and multiple linear regression and logistic regression analyses followed by the ROC curve. Results Children with ECC exhibited lower pH and BC in saliva and a higher CA VI ACT in both saliva and biofilm. Only saliva pH could predict the dmfs + active WSL. In biofilm, if CA VI ACT is increased by one pixel/µg, 0.85 ± 0.28 increase is expected in the number of active WSL. Salivary pH and CA VI ACT in saliva and biofilm had the power to predict ECC occurrence. Conclusion Changes in saliva pH and CA VI ACT in biofilm predicted the number of lesion occurrence. Furthermore, CA VI ACT in both saliva and biofilm can predict propensity for ECC.