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Evaluation of low fluoride toothpaste using primary enamel and a validated pH ‐cycling model
Author(s) -
Velo Marilia Mattar de Amoêdo Campos,
Tabchoury Cínthia Pereira Machado,
Romão Dayse Andrade,
Cury Jaime Aparecido
Publication year - 2016
Publication title -
international journal of paediatric dentistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.183
H-Index - 62
eISSN - 1365-263X
pISSN - 0960-7439
DOI - 10.1111/ipd.12209
Subject(s) - demineralization , enamel paint , fluoride , toothpaste , dentistry , medicine , remineralisation , nuclear chemistry , chemistry , inorganic chemistry
Aim To develop and validate pH ‐cycling model for primary enamel, which was then used to evaluate the anti‐caries potential of fluoride toothpastes. Design Human primary enamel slabs were subjected to pH ‐cycling model for 10 days and maintained for 6 h in demineralizing solution and 18 h in remineralizing solution daily. Twice/day, the slabs were treated. To validate it, the treatments were water or solutions containing 62.5, 125, 250, and 375 μ g F/mL. Commercial toothpastes containing no fluoride, 500, 1100, and 1450 μ g F/g were evaluated. Demineralization was assessed by percentage of surface hardness loss (% SHL ) and cross‐sectional hardness (ΔS). Fluoride dose–response effect was analysed by quadratic regression and the effects of toothpastes by Tukey's test. Results Dose–response effect was found between fluoride concentration and % SHL ( R 2 = 0.7047; P < 0.01) or ΔS ( R 2 = 0.4465; P < 0.01). % SHL and ΔS (mean ± SD ) for the group treated with 500 μ g F/g toothpaste was 36.6 ± 8.0 and 6298.5 ± 1221.3, respectively, which were significantly higher than those treated with 1100 (25.2 ± 8.7; 4565.7 ± 1122) and 1450 μ g F/g (24.2 ± 5.2; 2339.1 ± 879.7) toothpastes. Conclusion The developed pH ‐cycling model may be used to evaluate and compare the anti‐caries potential of toothpaste formulations with low fluoride concentration because it presents dose–response effects on the reduction of primary enamel demineralization.