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Analysis of the association between polymorphisms in MMP2 , MMP3 , MMP9 , MMP20 , TIMP1 , and TIMP2 genes with white spot lesions and early childhood caries
Author(s) -
Antunes Lívia Azeredo,
Antunes Leonardo Santos,
Küchler Erika Calvano,
Lopes Ludiana Barbosa,
Moura Alex,
Bigonha Rhyan Silva,
Abreu Fernanda Volpe,
Granjeiro José Mauro,
Amorim Lidia Maria da Fonte,
Paixão Izabel Christi. P.
Publication year - 2016
Publication title -
international journal of paediatric dentistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.183
H-Index - 62
eISSN - 1365-263X
pISSN - 0960-7439
DOI - 10.1111/ipd.12202
Subject(s) - medicine , genotype , genotyping , odds ratio , single nucleotide polymorphism , gastroenterology , genetics , biology , gene
Background Matrix metalloproteinases and their inhibitors might be involved in enamel formation. Aim This study aimed to evaluate the association between polymorphisms in MMP 2 , MMP 3 , MMP 9 , MMP 20 , TIMP 1 , and TIMP 2 with white spot lesions ( WSL ) and early childhood caries ( ECC ). Design A cross‐sectional study was performed on 786 children aged from 2 to 6 years in Brazil. After clinical evaluation, they were classified into groups with disease (the presence of WSL and/or ECC ) and without disease (the absence of WSL or ECC ). Genotyping of the selected polymorphisms was carried out with TaqMan real‐time PCR , using genomic DNA extracted from buccal cells. Allele and genotype frequencies were compared between groups. Chi‐square test, odds ratio ( OR ), and logistic regression were used ( P ≤ 0.05). Results The dmft score was 1.3 ( SD : 2.4), and 41.34% of the children have at least one caries lesion. In MMP 9 , the GG genotype was more frequent in the group without disease ( P = 0.006). In a recessive model, WSL was associated with the marker rs1711437 in MMP 20 ( P = 0.019; OR = 1.20, 95% CI 1.02–1.42). The marker rs1784418 in MMP 20 showed an association between the allele G distribution for the WSL group ( P = 0.020; OR = 0.73, 95% CI 0.55–0.96). Conclusion MMP 9 and MMP 20 are involved in WSL and ECC development.