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Effect of sealing infected dentin with glass ionomer cement on the abundance and localization of MMP‐2, MMP‐8, and MMP‐9 in young permanent molars in vivo
Author(s) -
Kuhn Eunice,
Reis Alessandra,
Campagnoli Eduardo Bauml,
Chibinski Ana Cláudia Rodrigues,
Carrilho Marcela Rocha de Oliveira,
Wambier Denise Stadler
Publication year - 2016
Publication title -
international journal of paediatric dentistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.183
H-Index - 62
eISSN - 1365-263X
pISSN - 0960-7439
DOI - 10.1111/ipd.12167
Subject(s) - medicine , glass ionomer cement , matrix metalloproteinase , dentin , molar , dentistry , in vivo , microbiology and biotechnology , biology
Background The study of MMPs’ behavior in carious lesions contributes to the understanding of the mechanisms involved in dentin reorganization after restoration. Aim To compare the abundance and localization of MMPs 2, 8, and 9 in infected dentin before and after restoration. Design The sample consisted of 23 young permanent molars with active deep carious lesions. Infected carious dentin samples were collected from the same tooth at baseline and 60 days after cavity lining with GIC and composite resin restoration and processed for immunohistochemistry assays. After digital images were obtained, two calibrated operators analyzed the samples according to the immunostaining intensity and the MMPs’ localization. Chi‐square test was used for statistical analysis. Results The intensity of immunostaining for MMP‐8 was reduced after 60 days ( P = 0.02), and no difference was observed for MMP‐2 ( P = 0.32) and MMP‐9 ( P = 0.14). The MMPs’ distribution was generalized in the intertubular dentin and absent or located in the intratubular dentin, regardless of the period. Conclusion The sealing of infected carious dentin in young permanent molars reduced the expression of MMP‐8, which is consistent with the initial remodeling process of the dentin matrix.