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Electrostatic dust collectors compared to inhalable samplers for measuring endotoxin concentrations in farm homes
Author(s) -
KilburgBasnyat B.,
Peters T. M.,
Perry S. S.,
Thorne P. S.
Publication year - 2016
Publication title -
indoor air
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.387
H-Index - 99
eISSN - 1600-0668
pISSN - 0905-6947
DOI - 10.1111/ina.12243
Subject(s) - limulus amebocyte lysate , particle counter , geometric standard deviation , environmental science , zoology , geometric mean , particulates , environmental chemistry , chemistry , bioaerosol , environmental engineering , aerosol , atmospheric sciences , mathematics , biology , physics , statistics , organic chemistry , lipopolysaccharide , endocrinology
Paired electrostatic dust collectors ( EDC s) and daily, inhalable button samplers ( BS ) were used concurrently to sample endotoxin in 10 farm homes during 7‐day periods in summer and winter. Winter sampling included an optical particle counter ( OPC ) to measure PM 2.5 and PM 2.5–10 . Electrostatic dust collectors and BS filters were analyzed for endotoxin using the kinetic chromogenic Limulus amebocyte lysate assay. Optical particle counter particulate matter ( PM ) data were divided into two PM categories. In summer, geometric mean (geometric standard deviation) endotoxin concentrations were 0.82 EU /m 3 (2.7) measured with the BS and 737 EU /m 2 (1.9) measured with the EDC . Winter values were 0.52 EU /m 3 (3.1) for BS and 538 EU /m 2 (3.0) for EDC s. Seven‐day endotoxin values of EDC s were highly correlated with the 7‐day BS sampling averages ( r = 0.70; P < 0.001). Analysis of variance indicated a 2.4‐fold increase in EDC endotoxin concentrations for each unit increase of the ratio of PM 2.5 to PM 2.5–10 . There was also a significant correlation between BS and EDC s endotoxin concentrations for winter ( r = 0.67; P < 0.05) and summer ( r = 0.75; P < 0.05). Thus, EDC s sample comparable endotoxin concentrations to BS , making EDC s a feasible, easy to use alternative to BS for endotoxin sampling.