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Methods for quantifying S taphylococcus aureus in indoor air
Author(s) -
Chang C.W.,
Wang L.J.
Publication year - 2015
Publication title -
indoor air
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.387
H-Index - 99
eISSN - 1600-0668
pISSN - 0905-6947
DOI - 10.1111/ina.12124
Subject(s) - staphylococcus aureus , chemistry , sampling time , chromatography , sampling (signal processing) , saline , cell counting , phosphate buffered saline , microbiology and biotechnology , bacteria , cell , biology , medicine , biochemistry , mathematics , cell cycle , statistics , genetics , filter (signal processing) , computer science , computer vision
S taphylococcus aureus has been detected in indoor air and linked to human infection. Quantifying S . aureus by efficient sampling methods followed by appropriate sample storage treatments is essential to characterize the exposure risk of humans. This laboratory study evaluated the effects of sampler type (all‐glass impinger ( AGI ‐30), BioSampler, and Andersen one‐stage sampler (Andersen 1‐ STG )), collection fluid (deionized water ( DW ), phosphate‐buffered saline ( PBS ), and Tween mixture ( TM )), and sampling time (3–60 min) on cell recovery. Effects of storage settings on bacterial concentration were also assessed over 48 h. Results showed BioSampler performed better than Andersen 1‐ STG and AGI ‐30 ( P < 0.05) and TM was superior to PBS and DW ( P < 0.05). An increase in sampling time negatively affected the recoveries of cells in PBS of BioSampler and AGI ‐30 ( P < 0.05), whereas cell recoveries in TM were increased at sampling of 6–15 min compared with 3 min. Concentrations of cells collected in PBS were decreased with storage time at 4 and 23°C (P < 0.05), while cells stored in TM showed stable concentrations at 4°C ( P > 0.05) and increased cell counts at 23°C ( P < 0.05). Overall, sampling by BioSampler with TM followed by sample transportation and storage at 4°C is recommended.