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Stability of airborne microbes in the L ouvre M useum over time
Author(s) -
Gaüzère C.,
MolettaDenat M.,
Blanquart H.,
Ferreira S.,
Moularat S.,
Godon J.J.,
Robine E.
Publication year - 2014
Publication title -
indoor air
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.387
H-Index - 99
eISSN - 1600-0668
pISSN - 0905-6947
DOI - 10.1111/ina.12053
Subject(s) - proteobacteria , actinobacteria , biology , chryseobacterium , pyrosequencing , microorganism , context (archaeology) , microbial population biology , firmicutes , microbiology and biotechnology , stenotrophomonas , microbial ecology , bacteria , ecology , pseudomonas , 16s ribosomal rna , genetics , gene , paleontology
The microbial content of air has as yet been little described, despite its public health implications, and there remains a lack of environmental microbial data on airborne microflora in enclosed spaces. In this context, the aim of this study was to characterize the diversity and dynamics of airborne microorganisms in the L ouvre M useum using high‐throughput molecular tools and to underline the microbial signature of indoor air in this human‐occupied environment. This microbial community was monitored for 6 month during occupied time. The quantitative results revealed variations in the concentrations of less than one logarithm, with average values of 10 3 and 10 4 Escherichia coli/Aspergillus fumigatus genome equivalent per m 3 for bacteria and fungi, respectively. Our observations highlight the stability of the indoor airborne bacterial diversity over time, while the corresponding eukaryote community was less stable. Bacterial diversity characterized by pyrosequencing 454 showed high diversity dominated by the P roteobacteria which represented 51.1%, 46.9%, and 38.4% of sequences, for each of the three air samples sequenced. A common bacterial diversity was underlined, corresponding to 58.4% of the sequences. The core species were belonging mostly to the Proteobacteria and Actinobacteria , and to the genus Paracoccus spp., Acinetobacter sp., Pseudomonas sp., Enhydrobacter sp., Sphingomonas sp., Staphylococcus sp., and Streptococcus sp.