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Siglec‐F is induced by granulocyte–macrophage colony‐stimulating factor and enhances interleukin‐4‐induced expression of arginase‐1 in mouse macrophages
Author(s) -
Tateyama Hiroyuki,
Murase Yusuke,
Higuchi Hiroshi,
Inasaka Yui,
Kaneoka Hidenori,
Iijima Shinji,
Nishijima Kenichi
Publication year - 2019
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/imm.13121
Subject(s) - siglec , biology , microbiology and biotechnology , stat5 , gene knockdown , stat protein , haematopoiesis , signal transduction , immunology , stat3 , immune system , cell culture , stem cell , genetics
Summary Siglecs are cell surface lectins that recognize sialic acids and are primarily expressed in hematopoietic cells. Previous studies showed that some Siglecs regulate macrophage function. In the present study, we examined the induction and putative roles of mouse Siglec‐F in bone‐marrow‐derived macrophages in mice. A quantitative RT ‐ PCR analysis showed that the basal expression of Siglec‐F was weak in bone‐marrow‐derived macrophages differentiated by macrophage colony‐stimulating factor. However, a 24‐hr stimulation with granulocyte–macrophage colony‐stimulating factor ( GM ‐ CSF ) enhanced Siglec‐F expression. GM ‐ CSF also enhanced Siglec‐F expression in thioglycollate‐induced peritoneal macrophages. The inhibition of signal transducer and activator of transcription 5 ( STAT 5), but not that of phosphoinositide 3‐kinase or mitogen‐activated protein kinase kinase, significantly reduced the induction of Siglec‐F. Interleukin‐3, which uses a common β ‐chain shared with the GM ‐ CSF receptor to stimulate the STAT 5 pathway, also enhanced Siglec‐F expression. The knockdown of Siglec‐F by a specific small interfering RNA enhanced GM ‐ CSF ‐induced STAT 5 phosphorylation, suggesting that Siglec‐F down‐regulates its own expression upon prolonged GM ‐ CSF stimulation. Furthermore, the knockdown of Siglec‐F reduced the STAT 6 phosphorylation and expression of arginase‐1 in interleukin‐4‐stimulated macrophages. These results suggest that Siglec‐F fine‐tunes the immune responses of macrophages.