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Peyer's patches‐derived CD11b + B cells recruit regulatory T cells through CXCL9 in dextran sulphate sodium‐induced colitis
Author(s) -
Wang Zhiming,
Zhang Hushan,
Liu Ronghua,
Qian Tingting,
Liu Jiajing,
Huang Enyu,
Lu Zhou,
Zhao Chujun,
Wang Luman,
Chu Yiwei
Publication year - 2018
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/imm.12977
Subject(s) - isotype , peyer's patch , chemokine , integrin alpha m , colitis , immunology , biology , microbiology and biotechnology , t cell , cxcl9 , chemistry , antibody , cxcl10 , immune system , monoclonal antibody
Summary Regulatory T (Treg) cells play an essential role in the maintenance of intestinal homeostasis. In Peyer's patches (PPs), which comprise the most important IgA induction site in the gut‐associated lymphoid tissue, Treg cells promote IgA isotype switching. However, the mechanisms underlying their entry into PPs and isotype switching facilitation in activated B cells remain unknown. This study, based on the dextran sulphate sodium (DSS)‐induced colitis model, revealed that Treg cells are significantly increased in PPs, along with CD11b + B‐cell induction. Immunofluorescence staining showed that infiltrated Treg cells were located around CD11b + B cells and produced transforming growth factor‐ β , thereby inducing IgA + B cells. Furthermore, in vivo and in vitro studies revealed that CD11b + B cells in PPs had the capacity to recruit Treg cells into PPs rather than promoting their proliferation. Finally, we found that Treg cell recruitment was mediated by the chemokine CXCL9 derived from CD11b + B cells in PPs. These findings demonstrate that CD11b + B cells induced in PPs during colitis actively recruit Treg cells to accomplish IgA isotype switch in a CXCL9‐dependent manner.