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Invariant natural killer T‐cell development and function with loss of micro RNA ‐155
Author(s) -
Frias Adolfo B.,
Buechel Heather M.,
Neupane Arpan,
D'Cruz Louise M.
Publication year - 2018
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/imm.12836
Subject(s) - dicer , biology , microbiology and biotechnology , microrna , cytokine , natural killer t cell , cell growth , cd1d , mir 155 , immunology , immune system , t cell , rna , rna interference , gene , genetics
Summary Invariant natural killer T ( iNKT ) cells are adaptive T cells with innate‐like characteristics including rapid cytokine production and a proliferative response to stimulation. Development of these cells in the thymus is dependent on expression of the micro RNA (mi RNA ) processing enzyme Dicer, indicating that iNKT cells probably have distinct mi RNA requirements for gene regulation during development. The mi RNA miR‐155 has previously been shown to have numerous roles in T cells, including regulation of proliferation and differentiation, and positive modulation of interferon‐ γ expression. We examined the role of miR‐155 in the development and function of iNKT cells. Using germline‐deficient miR‐155 mice, we showed that loss of miR‐155 resulted in unchanged iNKT cell frequency and cell number. Although miR‐155 was up‐regulated in iNKT cells upon activation with α ‐galactosylceramide, loss of miR‐155 did not affect cytokine production or proliferation by iNKT cells. Hence, cytokine production occurs in iNKT cells independently of miR‐155 expression.