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1,25‐dihydroxyvitamin D 3 ‐induced dendritic cells suppress experimental autoimmune encephalomyelitis by increasing proportions of the regulatory lymphocytes and reducing T helper type 1 and type 17 cells
Author(s) -
Xie Zhongxiang,
Chen Jingtao,
Zheng Chao,
Wu Jing,
Cheng Yun,
Zhu Shan,
Lin Chenhong,
Cao Qingqing,
Zhu Jie,
Jin Tao
Publication year - 2017
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/imm.12776
Subject(s) - experimental autoimmune encephalomyelitis , foxp3 , immunology , il 2 receptor , adoptive cell transfer , regulatory b cells , immune system , rar related orphan receptor gamma , spleen , innate lymphoid cell , regulatory t cell , biology , medicine , interleukin 10 , acquired immune system , t cell
Summary Dendritic cells ( DC s), a bridge for innate and adaptive immune responses, play a key role in the development of multiple sclerosis ( MS ) and experimental autoimmune encephalomyelitis ( EAE ), an animal model for MS . Administration of tolerogenic DC s has been used as an immunotherapy in autoimmune diseases. Deficiency of vitamin D is an environmental risk factor of MS . In this study, we induced tolerogenic DC s by 1,25‐dihydroxyvitamin D 3 and transferred the tolerogenic DC s ( VD 3 ‐ DC s) into EAE mice by adoptive transfer. We found that VD 3 ‐ DC s inhibited the infiltrations of T helper type 1 (Th1) and Th17 cells into spinal cord and increased the proportions of regulatory T cells ( CD 4 + CD 25 + Foxp3 + ), CD 4 + IL ‐10 + T cells and regulatory B cells ( CD 19 + CD 5 + CD 1d + ) in peripheral immune organs, which resulted in attenuated EAE . However, the proportions of T helper type 1 (Th1) and Th17 cells in spleen and lymph nodes and the levels of pro‐inflammatory cytokines and IgG in serum also increased after transfer of VD 3 ‐ DC s. We conclude that transfer of VD 3 ‐ DC s suppressed EAE by increasing proportions of regulatory T cells, CD 4 + IL ‐10 + T cells and regulatory B cells in spleen and reducing infiltration of Th1 and Th17 cells into spinal cord, which suggests a possible immunotherapy method using VD 3 ‐ DC s in MS .