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Peroxisome proliferator‐activated receptor‐ β / δ modulates mast cell phenotype
Author(s) -
Yao PeiLi,
Morales Jose L.,
Gonzalez Frank J.,
Peters Jeffrey M.
Publication year - 2017
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/imm.12699
Subject(s) - mast cell , peroxisome proliferator activated receptor , receptor , inflammation , biology , microbiology and biotechnology , immunology , biochemistry
Summary The peroxisome proliferator‐activated receptor‐ β / δ ( PPAR β / δ ) is known to have multiple anti‐inflammatory effects, typically observed in endothelial cells, macrophages, T cells and B cells. Despite the fact that mast cells are important mediators of inflammation, to date, the role of PPAR β / δ in mast cells has not been examined. Hence, the present study examined the hypothesis that PPAR β / δ modulates mast cell phenotype. Bone‐marrow‐derived mast cells ( BMMC s) and peritoneal mast cells from Pparβ/δ +/+ mice expressed higher levels of high‐affinity IgE receptor (Fc ε RI ) compared with Pparβ/δ −/− mice. BMMC s from Pparβ/δ +/+ mice also exhibited dense granules, associated with higher expression of enzymes and proteases compared with Pparβ/δ −/− mice. Resting BMMC s from Pparβ/δ +/+ mice secreted lower levels of inflammatory cytokines, associated with the altered activation of phospholipase C γ 1 and extracellular signal‐regulated kinases compared with Pparβ/δ −/− mice. Moreover, the production of cytokines by mast cells induced by various stimuli was highly dependent on PPAR β / δ expression. This study demonstrates that PPAR β / δ is an important regulator of mast cell phenotype.