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Detrimental effect of systemic antimicrobial CD 4 + T‐cell reactivity on gut epithelial integrity
Author(s) -
Kwong Chung Cheong K.C.,
Ronchi Francesca,
Geuking Markus B.
Publication year - 2017
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/imm.12682
Subject(s) - immune system , antimicrobial peptides , immunology , microbiology and biotechnology , antigen , intestinal epithelium , antimicrobial , biology , innate immune system , t cell , beta defensin , systemic inflammation , inflammation , epithelium , genetics
Summary Healthy host–microbe mutualism relies on compartmentalization and proper regulation of systemic and mucosal immune responses. Nevertheless, the systemic immune system is frequently exposed to bouts of bacteraemia, which can trigger systemic antimicrobial immune reactivity including CD 4 + T cells. Low‐level bacteraemia can occur when immune compartmentalization is compromised, for example in the presence of innate immune deficiency or following use of non‐steroidal anti‐inflammatory drugs. We generated an Escherichia coli strain expressing a defined T helper neo‐epitope to study systemic antigen‐specific antimicrobial CD 4 + T cells and their potential involvement in the pathogenisis of inflammatory bowel diseases. We found that the dose of bacteria required for the induction of systemic antimicrobial CD 4 + T‐cell proliferation was high and not easily reached under physiological conditions. Importantly, however, when intestinal barrier function was compromised by induced damage to the intestinal epithelium, the presence of systemic antimicrobial CD 4 + T cells specific for a single neo‐antigen resulted in dramatically increased levels of bacterial translocation. This study therefore demonstrates that systemic antimicrobial CD 4 + T‐cell reactivity might impact adversely on the mucosa under conditions of reduced barrier function and that despite strong mucosal immune regulation, antigen‐specific recognition is still sensitive.